Products 丨 DNA Analyzer​

Halo DNAmaster​

Halo DNAmaster DNA Analyzers

The Halo DNAmaster complements previous ranges of Halo spectrophotometers and plate readers. It is designed for measuring and analysing precious samples, requiring only microvolume quantities. It goes without saying that DNAmaster delivers reliability, precision and reproducibility.

The Halo DNAmaster is equipped with a comprehensive selection of on-board functions for versatility and is suitable for many life science applications involving nucleic acid, proteins and bacterial cultures.

DNA Master Main Menu

User Friendly Operation & Information Rich LCD Display

The 90mm x 120 mm, colour LCD screen is touch sensitive with intuitively designed software for quick selection by sample type. Effortless step-by-step navigation and a virtual QWERTY keyboard provide fast and efficient input and analysis.

Data is displayed numerically and in a graphical format (where applicable) with the further option of direct printing or storage in an SD card. Master Report PC software is available for converting the settings and results into a report format (Microsoft® Excel is required).

Nucleic Acid Analysis

The Halo DNAmaster is configured with onboard functions for the quantification of nucleic acids. Select the nucleic acid of choice such as double stranded DNA, single stranded DNA, RNA or oligonucleotides and absorbance measurements at the prescribed wavelength are directly converted into concentration units. The detection limit of the Halo DNAmaster is 6ng/μl (double stranded DNA), with optional 2mm cap of ultramicro cell.

The purity of nucleic acids can also be determined from ratio calculations such as A260 / A280 nm for protein contamination of DNA preparations.
Furthermore, The Halo DNAmaster can measure concentrations DNA, RNA and oligonucloetide – dye complexes.

Protein Analysis

Protein Analysis

The Halo DNAmaster measures protein concentrations from a range of colourimetric assays such as Bradford, Lowry, Biuret and BCA. In addition to numerical data the standard calibration curve can be displayed.

Furthermore, measure protein alone at 280nm or protein-dye complexes that absorb at different wavelengths.

Cell Culture Optical Density

The Halo DNAmaster also measures bacterial cell density at 600nm. Absorbance readings of approximately 0.4units define a bacterial culture in exponential growth phase and at the most appropriate time for harvest or induction.

Cell Culture Optical Density

Conventional Spectrophotometry

At the touch of a button the Halo DNAmaster converts into a conventional spectrophotometer for use with either the Ultramicro cell or a standard 10mm optical path length quartz or glass cuvette. Perform single wavelength photometry in either absorbance or % transmittance mode including multiple wavelength photometry for up to 6 user-defined wavelengths in absorbance mode.

Other functions include wavelength scans, time scans for kinetic studies and concentration calculations from standard curves.

Validation Functions

To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters is executed each time the Halo DNAmaster is switched on, including a selectable GLP/GMP function. (Validation kit is required in some parameter.)

Thoughtful Design​

Thoughtful Design

Dynamica considered every detail in the design of Halo DNAmaster, for example, the cuvette holder can be removed for washing and it is autoclavable for decontamination. Furthermore, a cover protects both the cuvette holder and the detector from dust and dirt when the unit is not in use. The side mounted cuvette rack is detachable for easy cleaning and a smaller footprint if bench space is at a premium.

Ultramicro Cell

The Ultramicro cell utilises fibre-optic technology and is designed for measuring and analysing extremely small volumes of DNA/RNA samples with high accuracy and outstanding reproducibility. In applications involving very high or very low DNA concentrations, a single light path is often insufficient, however the interchangeable cap of the Ultramicro cell imparts light paths of 1 mm and 0.2 mm respectively and effectively creates virtual dilutions to overcome such situations.

Other path lengths are available for different volume and concentration limits. A further function of the cap is to prevent the sample from drying up and to ensure that measurements remain reproducible since sample characteristics can be affected by evaporation of the solvent.

Ultramicro Cell
Programmable Temperature Control Model​

Programmable Temperature Control Model

The DNAmaster temperature control model utilises a peltier regulated cell holder to control the sample temperature from a range of +20˚C to +95˚C (with ±0.5˚C precision). Predominantly designed for the determination of nucleic acid Tm (at 260nm) up to 4 heating profiles can be selected, including 1˚C/min, 2˚C/min or a quick heat mode for heating up to a plateau temperature of 50˚C at 5˚C/min before ramping commences at 1˚C/min or 2˚C/min.

 

The heated cell holder can also be used in other applications requiring incubation and/or keeping a sample at a constant temperature, such as during kinetic analyses.

Specification

ModelHalo DNAmaster
Lamp SourceLong Life Xenon Flash Lamp
Detector Device2048 pixel CCD
Wavelength Range200-900nm
Measuring Range0-4.0 OD
Wavelength Accuracy+/-1nm
Bandwidth4nm
Noise~0.005 OD (RMS)
Drift~0.005 OD
Photometric Accuracy+/-0.01 OD
Photometric Repeatability+/-0.005 OD
Stray Light0.5%T
Ultramicro Cell dsDNA Detection Limit
(Depends on the Cap Used)
13ng/μl (Standard cap)
6ng/μl (Optional cap)
Ultramicro Cell Minimum Sample Volume (Depends on the Cap Used)0.7μl (Standard cap)
0.5μl (Optional cap)
Start Up MelodiesSelectable from 7 types and mute
Energy Saving ModeYes
Memory StorageInternal or SD card
Result Storage40,000 (internal)
Unlimited (SD Card)
User-Defined Program Storage10 for each function
Dimension W x D x H (mm)300 x 300 x 115
300 x 300 x 155 (TC model)
Weight (kg)3.5kg
4.5kg (TC model)
Power Requirement110 – 220 V, 50/60Hz

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